Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [4]
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Validation data
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- Product number
- PA5-47919 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ASAM Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.
- Reactivity
- Human, Mouse
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of mouse adipose (uterus) tissue and mouse brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-mouse ASAM Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47919) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for ASAM at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ASAM in mouse adipose (uterus) tissue and mouse brain (cortex) tissue. Samples were incubated in ASAM polyclonal antibody (Product # PA5-47919) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for ASAM at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of T98G human glioblastoma cell line was stained with Sheep Anti-mouse ASAM Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47919, filled histogram) or control antibody (open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of T98G human glioblastoma cell line was stained with Sheep Anti-mouse ASAM Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47919, filled histogram) or control antibody (open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of ASAM of RAW 264.7 mouse monocyte/macrophage cell line. Samples were incubated in ASAM polyclonal antibody (Product # PA5-47919) or control antibody followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of ASAM of T98G human glioblastoma cell line. Samples were incubated in ASAM polyclonal antibody (Product # PA5-47919) or control antibody followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody.