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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [2]
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- Product number
- AF7356 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse ASAM Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and mouse recombinant ASAM in direct ELISAs. Detects human and mouse ASAM in Western blots.
- Reactivity
- Human, Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q8R373- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse ASAM by Western Blot. Western blot shows lysates of mouse adipose (uterus) tissue and mouse brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse ASAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7356) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ASAM at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of ASAM in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Sheep Anti-Mouse ASAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7356, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of ASAM in T98G Human Cell Line by Flow Cytometry. T98G human glioblastoma cell line was stained with Sheep Anti-Mouse ASAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7356, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).
