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Western blot
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- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF2245 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal EPCR Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human EPCR in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse EPCR is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Interplay of Plasmodium falciparum and thrombin in brain endothelial barrier disruption.
Blood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytes.
Infected erythrocytes expressing DC13 PfEMP1 differ from recombinant proteins in EPCR-binding function.
Interaction between Endothelial Protein C Receptor and Intercellular Adhesion Molecule 1 to Mediate Binding of Plasmodium falciparum-Infected Erythrocytes to Endothelial Cells.
Cellular regulation of blood coagulation: a model for venous stasis.
Comprehensive analysis of conditioned media from ovarian cancer cell lines identifies novel candidate markers of epithelial ovarian cancer.
Aldosterone modifies hemostasis via upregulation of the protein-C receptor in human vascular endothelium.
Protein C is an autocrine growth factor for human skin keratinocytes.
Avril M, Benjamin M, Dols MM, Smith JD
Scientific reports 2019 Sep 11;9(1):13142
Scientific reports 2019 Sep 11;9(1):13142
Blood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytes.
Ecklu-Mensah G, Olsen RW, Bengtsson A, Ofori MF, Hviid L, Jensen ATR, Adams Y
PloS one 2018;13(10):e0204177
PloS one 2018;13(10):e0204177
Infected erythrocytes expressing DC13 PfEMP1 differ from recombinant proteins in EPCR-binding function.
Azasi Y, Lindergard G, Ghumra A, Mu J, Miller LH, Rowe JA
Proceedings of the National Academy of Sciences of the United States of America 2018 Jan 30;115(5):1063-1068
Proceedings of the National Academy of Sciences of the United States of America 2018 Jan 30;115(5):1063-1068
Interaction between Endothelial Protein C Receptor and Intercellular Adhesion Molecule 1 to Mediate Binding of Plasmodium falciparum-Infected Erythrocytes to Endothelial Cells.
Avril M, Bernabeu M, Benjamin M, Brazier AJ, Smith JD
mBio 2016 Jul 12;7(4)
mBio 2016 Jul 12;7(4)
Cellular regulation of blood coagulation: a model for venous stasis.
Campbell JE, Brummel-Ziedins KE, Butenas S, Mann KG
Blood 2010 Dec 23;116(26):6082-91
Blood 2010 Dec 23;116(26):6082-91
Comprehensive analysis of conditioned media from ovarian cancer cell lines identifies novel candidate markers of epithelial ovarian cancer.
Gunawardana CG, Kuk C, Smith CR, Batruch I, Soosaipillai A, Diamandis EP
Journal of proteome research 2009 Oct;8(10):4705-13
Journal of proteome research 2009 Oct;8(10):4705-13
Aldosterone modifies hemostasis via upregulation of the protein-C receptor in human vascular endothelium.
Ducros E, Berthaut A, Mirshahi SS, Faussat AM, Soria J, Agarwal MK, Mirshahi M
Biochemical and biophysical research communications 2008 Aug 22;373(2):192-6
Biochemical and biophysical research communications 2008 Aug 22;373(2):192-6
Protein C is an autocrine growth factor for human skin keratinocytes.
Xue M, Campbell D, Jackson CJ
The Journal of biological chemistry 2007 May 4;282(18):13610-6
The Journal of biological chemistry 2007 May 4;282(18):13610-6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human EPCR by Western Blot. Western blot shows lysates of human skin tissue, human placenta tissue, and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human EPCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2245) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EPCR at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- EPCR in Human Liver. EPCR was detected in immersion fixed paraffin-embedded sections of human liver using 1.7 µg/mL Goat Anti-Human EPCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2245) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
