Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- BAF2245 - Provider product page
- Provider
- R&D Systems
- Product name
- Human EPCR Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human EPCR in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant mouse EPCR is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
Q9UNN8
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Comprehensive analysis of conditioned media from ovarian cancer cell lines identifies novel candidate markers of epithelial ovarian cancer.
Gunawardana CG, Kuk C, Smith CR, Batruch I, Soosaipillai A, Diamandis EP
Journal of proteome research 2009 Oct;8(10):4705-13
Journal of proteome research 2009 Oct;8(10):4705-13
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- EPCR in Human Liver. EPCR was detected in immersion fixed paraffin-embedded sections of human liver array using Goat Anti-Human EPCR Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2245) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.