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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- HPA019661 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA019661, RRID:AB_1858215
- Product name
- Anti-TPR
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human TPR, Gene description: translocated promoter region, nuclear basket protein, Validated applications: WB, IHC, ICC, Uniprot ID: P12270, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references A quantitative map of nuclear pore assembly reveals two distinct mechanisms
Exploring Metabolic Adaptations to the Acidic Microenvironment of Osteosarcoma Cells Unveils Sphingosine 1-Phosphate as a Valuable Therapeutic Target
The human nucleoporin Tpr protects cells from RNA-mediated replication stress
Otsuka S, Tempkin J, Zhang W, Politi A, Rybina A, Hossain M, Kueblbeck M, Callegari A, Koch B, Morero N, Sali A, Ellenberg J
Nature 2023;613(7944):575-581
Nature 2023;613(7944):575-581
Exploring Metabolic Adaptations to the Acidic Microenvironment of Osteosarcoma Cells Unveils Sphingosine 1-Phosphate as a Valuable Therapeutic Target
Cortini M, Armirotti A, Columbaro M, Longo D, Di Pompo G, Cannas E, Maresca A, Errani C, Longhi A, Righi A, Carelli V, Baldini N, Avnet S
Cancers 2021;13(2):311
Cancers 2021;13(2):311
The human nucleoporin Tpr protects cells from RNA-mediated replication stress
Kosar M, Giannattasio M, Piccini D, Maya-Mendoza A, García-Benítez F, Bartkova J, Barroso S, Gaillard H, Martini E, Restuccia U, Ramirez-Otero M, Garre M, Verga E, Andújar-Sánchez M, Maynard S, Hodny Z, Costanzo V, Kumar A, Bachi A, Aguilera A, Bartek J, Foiani M
Nature Communications 2021;12(1)
Nature Communications 2021;12(1)
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Enhanced validation
- Submitted by
- klas2
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting TPR or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting TPR Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
- Sample type
- U-2 OS
- Primary Ab dilution
- 1:111
- Conjugate
- Horseradish Peroxidase
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:3000
- Knockdown/Genetic Approaches Application
- Western blot
