12569-1-AP
antibody from Proteintech Group
Targeting: SCFD1
C14orf163, KIAA0917, RA410, SLY1, STXBP1L2
Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- 12569-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#12569-1-AP, RRID:AB_2183266
- Product name
- SCFD1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated SCFD1 antibody (Cat. #12569-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Recruitment of PI4KIIIβ to the Golgi by ACBD3 is dependent on an upstream pathway of a SNARE complex and golgins.
Acetylation of SCFD1 regulates SNARE complex formation and autophagosome-lysosome fusion.
Formononetin alleviates Streptococcus suis infection by targeting suilysin.
Silibinin attenuates Streptococcus suis serotype 2 virulence by targeting suilysin.
Autophagic substrate clearance requires activity of the syntaxin-5 SNARE complex.
Direct interaction between the COG complex and the SM protein, Sly1, is required for Golgi SNARE pairing.
Stalder D, Yakunin I, Pereira C, Eden J, Gershlick DC
Molecular biology of the cell 2024 Feb 1;35(2):ar20
Molecular biology of the cell 2024 Feb 1;35(2):ar20
Acetylation of SCFD1 regulates SNARE complex formation and autophagosome-lysosome fusion.
Huang H, Ouyang Q, Mei K, Liu T, Sun Q, Liu W, Liu R
Autophagy 2023 Jan;19(1):189-203
Autophagy 2023 Jan;19(1):189-203
Formononetin alleviates Streptococcus suis infection by targeting suilysin.
Wang G, Liu H, Liu Y, Li H, Li Z, Shao G, Lv X
Microbial pathogenesis 2020 Oct;147:104388
Microbial pathogenesis 2020 Oct;147:104388
Silibinin attenuates Streptococcus suis serotype 2 virulence by targeting suilysin.
Shen X, Liu H, Li G, Deng X, Wang J
Journal of applied microbiology 2019 Feb;126(2):435-442
Journal of applied microbiology 2019 Feb;126(2):435-442
Autophagic substrate clearance requires activity of the syntaxin-5 SNARE complex.
Renna M, Schaffner C, Winslow AR, Menzies FM, Peden AA, Floto RA, Rubinsztein DC
Journal of cell science 2011 Feb 1;124(Pt 3):469-82
Journal of cell science 2011 Feb 1;124(Pt 3):469-82
Direct interaction between the COG complex and the SM protein, Sly1, is required for Golgi SNARE pairing.
Laufman O, Kedan A, Hong W, Lev S
The EMBO journal 2009 Jul 22;28(14):2006-17
The EMBO journal 2009 Jul 22;28(14):2006-17
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- A549 cells were subjected to SDS PAGE followed by western blot with 12569-1-AP(SCFD1 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using SCFD1 antibody 12569-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-SCFD1 (IP:12569-1-AP, 3ug; Detection:12569-1-AP 1:500) with HeLa cells lysate 2150ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human endometrial cancer using 12569-1-AP(SCFD1 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue