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Western blot
Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- PA5-31175 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CAR Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, H1299, HCT116. Predicted reactivity: Mouse (90%), Rat (90%), Rabbit (90%), Bovine (91%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.05 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CAR Polyclonal Antibody detects Coxsackie Adenovirus Receptor protein at cell membrane by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Coxsackie Adenovirus Receptor stained by CAR Polyclonal Antibody (Product # PA5-31175) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.
