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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-31175 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CAR Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, H1299, HCT116.
- Concentration
- 1.05 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CAR using 30 µg of A) A431 (B) HeLa and C) HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a CAR polyclonal antibody (Product # PA5-31175) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CAR Polyclonal Antibody (Product # PA5-31175). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CAR Polyclonal Antibody (Product # PA5-31175) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CAR Polyclonal Antibody detects Coxsackie Adenovirus Receptor protein at cell membrane by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Coxsackie Adenovirus Receptor stained by CAR Polyclonal Antibody (Product # PA5-31175) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using CAR (Product # PA5-31175) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
