Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
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Validation data
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- Product number
- MA5-24792 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- 14-3-3 eta Monoclonal Antibody (3G12)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat, Bovine, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3G12
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of 14-3-3 eta in neural tissue and cell lysates . Samples were incubated in 14-3-3 eta monoclonal antibody (Product # MA5-24792). Mouse mAb against 14-3-3n ( green). [1] protein standard, [2] Rat whole brain, [3] Mouse whole brain, [4] NIH/3T3, [5] Hek293, [6] HeLa, [7] SH-SY5Y, [8] C6 cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 14-3-3 eta in rat mixed neuron/glial cells using 14-3-3 eta monoclonal antibody (Product # MA5-24792), DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of 14-3-3 eta in HeLa cells. Samples were incubated in 14-3-3 eta monoclonal antibody (Product # MA5-24792) using a dilution of 1:1000. Antibody in red. Blue is DAPI staining of nuclear DNA. The antibody reveals the diffuse cytoplasmic distribution of 14.3.3 protein with higher concentration in the perinuclear region.