PA5-118366

antibody from Invitrogen Antibodies

Targeting: YWHAH YWHA1

Western blot (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118366

Provider

Invitrogen Antibodies

Product name

14-3-3 eta Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

1.0 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

14-3-3 eta Immunoprecipitation using: Lane A: 0.5 mg A375 Whole Cell Lysate 4 µL with 14-3-3 eta Polyclonal Antibody (Product # PA5-118366) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: 14-3-3 eta Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 28 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of 14-3-3 eta in Lane A: 0.5 mg A375 Whole Cell Lysate. Samples were treated with 60 μg of immunomagnetic Protein A/G beads, incubated with polyclonal antibody (Product # PA5-118366) with a dilution of 1:100 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 28 kDa , Observed band size : 28 kDa .

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of 14-3-3 eta in Lane A: 0.5 mg A375 Whole Cell Lysate. Samples were treated with 60 μg of immunomagnetic Protein A/G beads, incubated with polyclonal antibody (Product # PA5-118366) with a dilution of 1:100 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 28 kDa , Observed band size : 28 kDa .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

14-3-3 eta Immunoprecipitation using: Lane A: 0.5 mg A375 Whole Cell Lysate 4 µL with 14-3-3 eta Polyclonal Antibody (Product # PA5-118366) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: 14-3-3 eta Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 28 kDa.