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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AF4420 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat 14-3-3 eta Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human, mouse, and rat 14-3-3 eta in Western blots. In Western Blot less than 1% cross reactivity with other recombinant human 14-3-3 isoforms.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q04917- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human/Mouse/ Rat 14-3-3 eta by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/ Mouse/Rat 14-3-3 eta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4420) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human 14-3-3 beta, epsilon, eta, gamma, sigma, theta, and zeta (2 ng/lane) were included. A specific band for 14-3-3 eta was detected at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
