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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-40876 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZFP36 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: SGPWSLSPSD SSPSGVTSRL PGRSTSLVEG RSCGWVPPPP GFAPLAPRLG Sequence homology: Human: 100%; Mouse: 92%; Rat: 92%; Yeast: 75%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human PANC1 cell lysate using an anti-ZFP36 polyclonal antibody (Product # PA5-40876).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using anti-ZFP36 Polyclonal Antibody (Product # PA5-40876) and a ~36 kDa band was observed across the panel tested. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1) and Hep G2 treated with Resveratrol (200ng/mL 12 hours) (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of human thymus tissue using an anti-ZFP36 polyclonal antibody (Product # PA5-40876).
