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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Flow cytometry [3]
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Validation data
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- Product number
- TA502378 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502378, RRID:AB_11126949
- Product name
- ZFP36 mouse monoclonal antibody, clone OTI4B10 (formerly 4B10)
- Antibody type
- Monoclonal
- Description
- ZFP36 mouse monoclonal antibody, clone OTI4B10 (formerly 4B10)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ZFP36
- Isotype
- IgG
- Antibody clone number
- OTI4B10
- Vial size
- 100 µl
- Concentration
- 0.68 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZFP36 (RC202049, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZFP36.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ZFP36 monoclonal antibody.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC202049 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ZFP36 antibody(TA502378), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-ZFP36 antibody(TA502378),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-ZFP36 antibody(TA502378),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
