PAB9949

antibody from Abnova Corporation

Targeting: BIVM FLJ20159

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB9949

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB9949, RRID:AB_1672168

Product name

BIVM polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of BIVM.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

BIVM, a novel gene widely distributed among deuterostomes, shares a core sequence with an unusual gene in Giardia lamblia.

Yoder JA, Hawke NA, Eason DD, Mueller MG, Davids BJ, Gillin FD, Litman GW
Genomics 2002 Jun;79(6):750-5

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot of BIVM polyclonal antibody (Cat # PAB9949).Whole cell HeLa lysate was used to probe for endogenous BIVM using Rabbit-anti-Human BIVM (C-terminal specific) polyclonal antibody.A 57 kDa band corresponding to human BIVM protein is detected using a 1 : 1,000 dilution of the antiserum incubated for 1 hour at room temperature.Washes with 1% Tween-20 TBS preceded reaction with HRP Gt-a-Rabbit IgG (H&L) diluted 1 : 10,000 and incubated for 1 hour at room temperature.The blot was developed using a chemiluminescent detection method (AP ECL 60 sec followed by a 45 sec exposure).