HPA003740

antibody from Atlas Antibodies

Targeting: CD180 LY64, Ly78, RP105

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA003740

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA003740, RRID:AB_1078450

Product name

Anti-CD180

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

DFQNNAIHYISREDMRSLEQAINLSLNFNGNNVKG
IELGAFDSTVFQSLNFGGTPNLSVIFNGLQNSTTQ
SLWLGTFEDIDDEDISSAMLKGLCEMSVESLNLQE
HRFSDISSTTFQCFTQLQELDLTATHLKGLPSGMK
GLNLLKKL


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human tonsil and cerebral cortex tissues using Anti-CD180 antibody. Corresponding CD180 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in white pulp.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows low expression as expected.

Sample type

HUMAN