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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- HPA024663 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA024663, RRID:AB_1857224
- Product name
- Anti-GINS4
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human GINS4, Gene description: GINS complex subunit 4 (Sld5 homolog), Alternative Gene Names: MGC14799, SLD5, Validated applications: ICC, IHC, WB, Uniprot ID: Q9BRT9, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Sld5 Ensures Centrosomal Resistance to Congression Forces by Preserving Centriolar Satellites
Kaur M, Devi R, Ghosh T, Khan M, Kumar P, Priyanka , Kar A, Sharma A, Varshney A, Kumar V, Saxena S
Molecular and Cellular Biology 2023;38(2)
Molecular and Cellular Biology 2023;38(2)
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and GINS4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403156).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & centrosome.
- Sample type
- Human
