MA5-17169

antibody from Invitrogen Antibodies

Targeting: RUNX3 AML2, CBFA3, PEBP2A3

Western blot

ELISA

Immunohistochemistry

Antibody data

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Validation data

Product number: MA5-17169 - Provider product page
Provider: Invitrogen Antibodies
Product name: RUNX3 Monoclonal Antibody (2B3)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-17169 targets RUNX3 in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human and Mouse samples. The MA5-17169 immunogen is purified recombinant fragment of human RUNX3 (amino acids:186-252) expressed in E. Coli. MA5-17169 detects RUNX3 which has a predicted molecular weight of approximately 44.4kDa.
Reactivity: Human, Mouse
Host: Mouse
Isotype: IgG
Antibody clone number: 2B3
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RUNX3 protein structure - MA5-17169 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of RUNX3 was performed using 70% confluent log phase SW480 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with RUNX3 Monoclonal Antibody (2B3) (Product # MA5-17169) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32766, 1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nucleus and cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of RUNX3 was performed using 70% confluent log phase SW480 and Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with RUNX3 Monoclonal Antibody (2B3) (Product # MA5-17169) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32766, 1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nucleus and cytoplasmic localization. Panel e represents Hep G2 showing no signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.