Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- 13-9817-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-RUNX3 Monoclonal Antibody (R3-5G4), Biotin, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This R3-5G4 monoclonal antibody recognizes human Runx3, a 44 kDa transcription factor that belongs to the Runt domain-containing family. Runx3 is expressed in hematopoietic cells such as T cells, B cells, and myeloid cells. This transcription factor is also expressed in non-hematopoietic tissues, including the epidermis, bone, and dorsal root ganglia. Runx3 has been established to play a critical role in thymocyte development. More specifically, Runx3 promotes CD8 + T cell development by silencing Cd4 expression. Finally, studies have demonstrated the tumor suppressive activity of in a variety of cancers (e.g., gastric and breast cancers). Applications Reported: This R3-5G4 antibody has been reported for use in western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This R3-5G4 antibody has been tested by immunohistochemistry on human FFPE tissue sections using high pH antigen retrieval at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- R3-5G4
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry of formalin-fixed paraffin embedded skin using 20 µg/mL Mouse IgG1 K Isotype Control Biotin (left) or 20 µg/mL Anti-Human Runx3 Biotin (right) followed by Streptavidin HRP and DAB visualization. Nuclei are counterstained with hematoxylin.
- Conjugate
- Biotin