14-9817-80

antibody from Invitrogen Antibodies
Targeting: RUNX3 AML2, CBFA3, PEBP2A3
Provider product page for 14-9817-80
Western blot
Immunohistochemistry

Antibody data

Product number
14-9817-80 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-RUNX3 Monoclonal Antibody (R3-5G4), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: This R3-5G4 monoclonal antibody recognizes human Runx3, a 44 kDa transcription factor that belongs to the Runt domain-containing family. Runx3 is expressed in hematopoietic cells such as T cells, B cells, and myeloid cells. This transcription factor is also expressed in non-hematopoietic tissues, including the epidermis, bone, and dorsal root ganglia. Runx3 has been established to play a critical role in thymocyte development. More specifically, Runx3 promotes CD8 + T cell development by silencing Cd4 expression. Finally, studies have demonstrated the tumor suppressive activity of in a variety of cancers (e.g., gastric and breast cancers). Applications Reported: This R3-5G4 antibody has been reported for use in western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This R3-5G4 antibody has been tested by western blotting of the Raji cell line and by immunohistochemistry of formalin-fixed paraffin embedded human skin tissue using the IHC Antigen Retrieval Solution - High pH (cat 00-4956). This can be used at less than or equal to 5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
R3-5G4
Vial size
25 µg
Concentration
0.5 mg/mL
Storage
4° C
RUNX3 protein structure - 14-9817-80 shown in red.