Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- 14-9817-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-RUNX3 Monoclonal Antibody (R3-5G4), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This R3-5G4 monoclonal antibody recognizes human Runx3, a 44 kDa transcription factor that belongs to the Runt domain-containing family. Runx3 is expressed in hematopoietic cells such as T cells, B cells, and myeloid cells. This transcription factor is also expressed in non-hematopoietic tissues, including the epidermis, bone, and dorsal root ganglia. Runx3 has been established to play a critical role in thymocyte development. More specifically, Runx3 promotes CD8 + T cell development by silencing Cd4 expression. Finally, studies have demonstrated the tumor suppressive activity of in a variety of cancers (e.g., gastric and breast cancers). Applications Reported: This R3-5G4 antibody has been reported for use in western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This R3-5G4 antibody has been tested by western blotting of the Raji cell line and by immunohistochemistry of formalin-fixed paraffin embedded human skin tissue using the IHC Antigen Retrieval Solution - High pH (cat 00-4956). This can be used at less than or equal to 5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- R3-5G4
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references RUNX3 acts as a tumor suppressor in breast cancer by targeting estrogen receptor α.
The Runx3 transcription factor augments Th1 and down-modulates Th2 phenotypes by interacting with and attenuating GATA3.
RUNX3, a novel tumor suppressor, is frequently inactivated in gastric cancer by protein mislocalization.
Runx3 and Runx1 are required for CD8 T cell development during thymopoiesis.
The RUNX3 gene--sequence, structure and regulated expression.
Huang B, Qu Z, Ong CW, Tsang YH, Xiao G, Shapiro D, Salto-Tellez M, Ito K, Ito Y, Chen LF
Oncogene 2012 Jan 26;31(4):527-34
Oncogene 2012 Jan 26;31(4):527-34
The Runx3 transcription factor augments Th1 and down-modulates Th2 phenotypes by interacting with and attenuating GATA3.
Kohu K, Ohmori H, Wong WF, Onda D, Wakoh T, Kon S, Yamashita M, Nakayama T, Kubo M, Satake M
Journal of immunology (Baltimore, Md. : 1950) 2009 Dec 15;183(12):7817-24
Journal of immunology (Baltimore, Md. : 1950) 2009 Dec 15;183(12):7817-24
RUNX3, a novel tumor suppressor, is frequently inactivated in gastric cancer by protein mislocalization.
Ito K, Liu Q, Salto-Tellez M, Yano T, Tada K, Ida H, Huang C, Shah N, Inoue M, Rajnakova A, Hiong KC, Peh BK, Han HC, Ito T, Teh M, Yeoh KG, Ito Y
Cancer research 2005 Sep 1;65(17):7743-50
Cancer research 2005 Sep 1;65(17):7743-50
Runx3 and Runx1 are required for CD8 T cell development during thymopoiesis.
Woolf E, Xiao C, Fainaru O, Lotem J, Rosen D, Negreanu V, Bernstein Y, Goldenberg D, Brenner O, Berke G, Levanon D, Groner Y
Proceedings of the National Academy of Sciences of the United States of America 2003 Jun 24;100(13):7731-6
Proceedings of the National Academy of Sciences of the United States of America 2003 Jun 24;100(13):7731-6
The RUNX3 gene--sequence, structure and regulated expression.
Bangsow C, Rubins N, Glusman G, Bernstein Y, Negreanu V, Goldenberg D, Lotem J, Ben-Asher E, Lancet D, Levanon D, Groner Y
Gene 2001 Nov 28;279(2):221-32
Gene 2001 Nov 28;279(2):221-32
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry on formalin-fixed paraffin embedded human skin using 5 µg/mL Mouse IgG1 Isotype Control (left) or 5 µg/mL Anti-Human Runx3 Purified (right) followed by Anti-Mouse IgG and DAB visualization.Nuclei are counterstained with hematoxylin.