Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- 14-9817-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-RUNX3 Monoclonal Antibody (R3-5G4), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This R3-5G4 monoclonal antibody recognizes human Runx3, a 44 kDa transcription factor that belongs to the Runt domain-containing family. Runx3 is expressed in hematopoietic cells such as T cells, B cells, and myeloid cells. This transcription factor is also expressed in non-hematopoietic tissues, including the epidermis, bone, and dorsal root ganglia. Runx3 has been established to play a critical role in thymocyte development. More specifically, Runx3 promotes CD8 + T cell development by silencing Cd4 expression. Finally, studies have demonstrated the tumor suppressive activity of in a variety of cancers (e.g., gastric and breast cancers). Applications Reported: This R3-5G4 antibody has been reported for use in western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This R3-5G4 antibody has been tested by western blotting of the Raji cell line and by immunohistochemistry of formalin-fixed paraffin embedded human skin tissue using the IHC Antigen Retrieval Solution - High pH (cat 00-4956). This can be used at less than or equal to 5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- R3-5G4
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references RUNX3 acts as a tumor suppressor in breast cancer by targeting estrogen receptor α.
The Runx3 transcription factor augments Th1 and down-modulates Th2 phenotypes by interacting with and attenuating GATA3.
RUNX3, a novel tumor suppressor, is frequently inactivated in gastric cancer by protein mislocalization.
Runx3 and Runx1 are required for CD8 T cell development during thymopoiesis.
The RUNX3 gene--sequence, structure and regulated expression.
Huang B, Qu Z, Ong CW, Tsang YH, Xiao G, Shapiro D, Salto-Tellez M, Ito K, Ito Y, Chen LF
Oncogene 2012 Jan 26;31(4):527-34
Oncogene 2012 Jan 26;31(4):527-34
The Runx3 transcription factor augments Th1 and down-modulates Th2 phenotypes by interacting with and attenuating GATA3.
Kohu K, Ohmori H, Wong WF, Onda D, Wakoh T, Kon S, Yamashita M, Nakayama T, Kubo M, Satake M
Journal of immunology (Baltimore, Md. : 1950) 2009 Dec 15;183(12):7817-24
Journal of immunology (Baltimore, Md. : 1950) 2009 Dec 15;183(12):7817-24
RUNX3, a novel tumor suppressor, is frequently inactivated in gastric cancer by protein mislocalization.
Ito K, Liu Q, Salto-Tellez M, Yano T, Tada K, Ida H, Huang C, Shah N, Inoue M, Rajnakova A, Hiong KC, Peh BK, Han HC, Ito T, Teh M, Yeoh KG, Ito Y
Cancer research 2005 Sep 1;65(17):7743-50
Cancer research 2005 Sep 1;65(17):7743-50
Runx3 and Runx1 are required for CD8 T cell development during thymopoiesis.
Woolf E, Xiao C, Fainaru O, Lotem J, Rosen D, Negreanu V, Bernstein Y, Goldenberg D, Brenner O, Berke G, Levanon D, Groner Y
Proceedings of the National Academy of Sciences of the United States of America 2003 Jun 24;100(13):7731-6
Proceedings of the National Academy of Sciences of the United States of America 2003 Jun 24;100(13):7731-6
The RUNX3 gene--sequence, structure and regulated expression.
Bangsow C, Rubins N, Glusman G, Bernstein Y, Negreanu V, Goldenberg D, Lotem J, Ben-Asher E, Lancet D, Levanon D, Groner Y
Gene 2001 Nov 28;279(2):221-32
Gene 2001 Nov 28;279(2):221-32
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry on formalin-fixed paraffin embedded human skin using 5 µg/mL Mouse IgG1 Isotype Control (left) or 5 µg/mL Anti-Human Runx3 Purified (right) followed by Anti-Mouse IgG and DAB visualization.Nuclei are counterstained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation (ChIP) assay of endogenous RUNX3 protein using Anti-RUNX3 Antibody: ChIP was performed using Anti-RUNX3 Monoclonal Antibody (R3-5G4) (Product # 14-9817-82) 5 µg, on sheared chromatin from HCT116 cells using the MAGnify ChIP System kit (Product # 49-2024). Normal Mouse IgG was used as a negative IP control. The purified DNA was analyzed by qPCR using primers binding to EZH2 promoter and SAT2 satellite repeats. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.