Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-52157 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Gemin 3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MAKLKHFHCR VLISTDLTSR GIDAEKVNLV VNLDVPLDWE TYMHRIGRAG RFGTLGLTVT YCCRGEEENM MMRIAQKCNI NLLPLPDPIP SGLMEECVDW DVEVKAAVHT YGIASVPNQP LKKQIQKIER TLQIQKAHGD H Highest antigen sequence identity to the following orthologs: Mouse - 83%, Rat - 84%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Gemin 3 in Lane 1: Marker (kDa) 220, 112, 84, 47, 32, 26, 17; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a Gemin 3 Polyclonal Antibody (Product # PA5-52157).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Gemin 3 in human cell line U-2 OS using a Gemin 3 Polyclonal Antibody (Product # PA5-52157) shows localization to nucleus, nuclear bodies and cytosol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Gemin 3 in human cell line U-2 OS shows positivity in nucleus & cytoplasm. Samples were probed using a Gemin 3 Polyclonal Antibody (Product # PA5-52157).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Gemin 3 in human testis and skeletal muscle tissues using Gemin 3 Polyclonal Antibody (Product # PA5-52157). Corresponding DDX20 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Gemin 3 in human testis using Gemin 3 Polyclonal Antibody (Product # PA5-52157) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Gemin 3 in human skeletal muscle using Gemin 3 Polyclonal Antibody (Product # PA5-52157) shows low expression as expected.
