TA500524

antibody from OriGene

Targeting: SATB1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500524

Provider

OriGene

Proper citation

OriGene Cat#TA500524, RRID:AB_2184451

Product name

Anti-SATB1 mouse monoclonal antibody, clone OTI4E1 (formerly 4E1)

Antibody type

Monoclonal

Description

Anti-SATB1 mouse monoclonal antibody, clone OTI4E1 (formerly 4E1)

Host

Mouse

Conjugate

Unconjugated

Epitope

SATB1

Isotype

IgG

Antibody clone number

OTI4E1

Vial size

100 µl

Concentration

0.79 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SATB1 (RC200421, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SATB1.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-SATB1 mouse monoclonal antibody (TA500524) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SATB1(RC200421).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-SATB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500524, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-SATB1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500524, Dilution 1:50)

Validation comment

IHC