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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- TA500589 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500589, RRID:AB_11140101
- Product name
- SATB1 mouse monoclonal antibody, clone OTI11E8 (formerly 11E8)
- Antibody type
- Monoclonal
- Description
- SATB1 mouse monoclonal antibody, clone OTI11E8 (formerly 11E8)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SATB1
- Isotype
- IgG
- Antibody clone number
- OTI11E8
- Vial size
- 100 µl
- Concentration
- 0.7 mg/ml
Submitted references SATB1 Level Correlates with Ki-67 Expression and Is a Positive Prognostic Factor in Non-small Cell Lung Carcinoma.
Glatzel-Plucinska N, Piotrowska A, Grzegrzolka J, Olbromski M, Rzechonek A, Dziegiel P, Podhorska-Okolow M
Anticancer research 2018 Feb;38(2):723-736
Anticancer research 2018 Feb;38(2):723-736
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SATB1 (RC200421, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SATB1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-SATB1 mouse monoclonal antibody (TA500589) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SATB1(RC200421).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SATB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500589)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SATB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500589)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-SATB1 antibody(TA500589),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
