HPA021649

antibody from Atlas Antibodies

Targeting: DNAI1 CILD1, DIC1, PCD

Provider product page for HPA021649

Western blot

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [14]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [1]

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Product number: HPA021649 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA021649, RRID:AB_1847913
Product name: Anti-DNAI1
Antibody type: Polyclonal
Description: Polyclonal Antibody against Human DNAI1, Gene description: dynein, axonemal, intermediate chain 1, Alternative Gene Names: CILD1, DIC1, PCD, Validated applications: WB, IHC, Uniprot ID: Q9UI46, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 100 µl
Concentration: 0.2 mg/ml
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Handling: The antibody solution should be gently mixed before use.

DNAI1 protein structure - HPA021649 shown in red.

Submitted references Shorter Cilia Length and Aberrant Ciliated Marker DNAI1 in Allergic Rhinitis

Zhou S, Liu Y, Yang Y, Huang H, Qiu Q
Journal of Inflammation Research 2023;Volume 16

Influenza virus reduces ubiquitin E3 ligase MARCH10 expression to decrease ciliary beat frequency.

Tsai M, Rayner RE, Chafin L, Farkas D, Adair J, Mishan C, Mallampalli RK, Kim SH, Cormet-Boyaka E, Londino JD
American journal of physiology. Lung cellular and molecular physiology 2023 May 1;324(5):L666-L676

CFAP300 mutation causing primary ciliary dyskinesia in Finland.

Schultz R, Elenius V, Fassad MR, Freke G, Rogers A, Shoemark A, Koistinen T, Mohamed MA, Lim JSY, Mitchison HM, Sironen AI
Frontiers in genetics 2022;13:985227

Identification of a novel founder variant in DNAI2 cause primary ciliary dyskinesia in five consanguineous families derived from a single tribe descendant of Arabian Peninsula.

Al-Mutairi DA, Alsabah BH, Alkhaledi BA, Pennekamp P, Omran H
Frontiers in genetics 2022;13:1017280

A quantitative super-resolution imaging toolbox for diagnosis of motile ciliopathies

Liu Z, Nguyen Q, Guan Q, Albulescu A, Erdman L, Mahdaviyeh Y, Kang J, Ouyang H, Hegele R, Moraes T, Goldenberg A, Dell S, Mennella V
Science Translational Medicine 2020;12(535)

TTC12 Loss-of-Function Mutations Cause Primary Ciliary Dyskinesia and Unveil Distinct Dynein Assembly Mechanisms in Motile Cilia Versus Flagella

Thomas L, Bouhouche K, Whitfield M, Thouvenin G, Coste A, Louis B, Szymanski C, Bequignon E, Papon J, Castelli M, Lemullois M, Dhalluin X, Drouin-Garraud V, Montantin G, Tissier S, Duquesnoy P, Copin B, Dastot F, Couvet S, Barbotin A, Faucon C, Honore I, Maitre B, Beydon N, Tamalet A, Rives N, Koll F, Escudier E, Tassin A, Touré A, Mitchell V, Amselem S, Legendre M
The American Journal of Human Genetics 2020;106(2):153-169

Genetic interaction between central pair apparatus genes CFAP221, CFAP54, and SPEF2 in mouse models of primary ciliary dyskinesia

McKenzie C, Lee L
Scientific Reports 2020;10(1)

CFAP45 deficiency causes situs abnormalities and asthenospermia by disrupting an axonemal adenine nucleotide homeostasis module.

Dougherty GW, Mizuno K, Nöthe-Menchen T, Ikawa Y, Boldt K, Ta-Shma A, Aprea I, Minegishi K, Pang YP, Pennekamp P, Loges NT, Raidt J, Hjeij R, Wallmeier J, Mussaffi H, Perles Z, Elpeleg O, Rabert F, Shiratori H, Letteboer SJ, Horn N, Young S, Strünker T, Stumme F, Werner C, Olbrich H, Takaoka K, Ide T, Twan WK, Biebach L, Große-Onnebrink J, Klinkenbusch JA, Praveen K, Bracht DC, Höben IM, Junger K, Gützlaff J, Cindrić S, Aviram M, Kaiser T, Memari Y, Dzeja PP, Dworniczak B, Ueffing M, Roepman R, Bartscherer K, Katsanis N, Davis EE, Amirav I, Hamada H, Omran H
Nature communications 2020 Nov 2;11(1):5520

TMEM16A chloride channel does not drive mucus production

Simões F, Quaresma M, Clarke L, Silva I, Pankonien I, Railean V, Kmit A, Amaral M
Life Science Alliance 2019;2(6):e201900462

Mutations in DNAH17, Encoding a Sperm-Specific Axonemal Outer Dynein Arm Heavy Chain, Cause Isolated Male Infertility Due to Asthenozoospermia

Whitfield M, Thomas L, Bequignon E, Schmitt A, Stouvenel L, Montantin G, Tissier S, Duquesnoy P, Copin B, Chantot S, Dastot F, Faucon C, Barbotin A, Loyens A, Siffroi J, Papon J, Escudier E, Amselem S, Mitchell V, Touré A, Legendre M
The American Journal of Human Genetics 2019;105(1):198-212

Mandatory role of HMGA1 in human airway epithelial normal differentiation and post-injury regeneration

Zhang H, Yang J, Walters M, Staudt M, Strulovici-Barel Y, Salit J, Mezey J, Leopold P, Crystal R
Oncotarget 2018;9(18):14324-14337

DNAH11 Localization in the Proximal Region of Respiratory Cilia Defines Distinct Outer Dynein Arm Complexes

Dougherty G, Loges N, Klinkenbusch J, Olbrich H, Pennekamp P, Menchen T, Raidt J, Wallmeier J, Werner C, Westermann C, Ruckert C, Mirra V, Hjeij R, Memari Y, Durbin R, Kolb-Kokocinski A, Praveen K, Kashef M, Kashef S, Eghtedari F, Häffner K, Valmari P, Baktai G, Aviram M, Bentur L, Amirav I, Davis E, Katsanis N, Brueckner M, Shaposhnykov A, Pigino G, Dworniczak B, Omran H
American Journal of Respiratory Cell and Molecular Biology 2016;55(2):213-224

Activation of NOTCH1 or NOTCH3 Signaling Skews Human Airway Basal Cell Differentiation toward a Secretory Pathway

Warburton D, Gomi K, Arbelaez V, Crystal R, Walters M
PLOS ONE 2015;10(2):e0116507

Generation of a human airway epithelium derived basal cell line with multipotent differentiation capacity

Walters M, Gomi K, Ashbridge B, Moore M, Arbelaez V, Heldrich J, Ding B, Rafii S, Staudt M, Crystal R
Respiratory Research 2013;14(1)

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Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Recombinant expression validation
Main image
Experimental details: Western blot analysis in control (vector only transfected HEK293T lysate) and dNAI1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402158).
Sample type: Human
Protocol: Protocol

Supportive validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Immunohistochemistry analysis in human testis and prostate tissues using HPA021649 antibody. Corresponding DNAI1 RNA-seq data are presented for the same tissues.
Sample type: Human
Protocol: Protocol
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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