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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-96455 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SND1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MVLSGCAIIV RGQPRGGPPP ERQINLSNIR AGNLARRAAA TQPDAKDTPD EPWAFPAREF LRKKLIGKEV CFTIENKTPQ GREYGMIYLG KDTNGENIAE SLVAEGLATR REGMRANNPE QNRLSECEEQ AKAAKKGMWS EGNGSHTIRD LKYTIENPRH FVDSHHQKPV NAIIEHVRDG SVVRALLLPD YYLVTVMLSG IKCPTFRREA DGSETPEPFA AEAKFFTESR LLQRDVQIIL ESCHNQNILG TILHPNGNIT; Positive Samples: HeLa, Jurkat, HL-60, MCF7, 293T, Mouse liver, Rat liver; Cellular Location: Cytoplasm, Melanosome, Nucleus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.33 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SND1 in extracts of various cell lines using SND1 Polyclonal Antibody (Product # PA5-96455) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SND1 in extracts of various cell lines using SND1 Polyclonal Antibody (Product # PA5-96455) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SND1 was performed in A-549 cells using SND1 Polyclonal Antibody (Product # PA5-96455). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SND1 was performed in NIH/3T3 cells using SND1 Polyclonal Antibody (Product # PA5-96455) at a dilution of 1:100. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SND1 in paraffin-embedded human kidney cancer using SND1 Polyclonal Antibody (Product # PA5-96455) at a dilution of 1:100.
