Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- PA5-76443 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RNaseL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RNaseL in extracts of various cells. Samples were incubated with RNaseL polyclonal antibody (Product # PA5-76443).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using RNaseL Polyclonal Antibody (Product # PA5-76443) and a ~85 kDa band corresponding to RNase L was observed across all cell lines and tissues. Whole cell extracts (30 µg lysate) of HL-60 (Lane 1), HEL 92.1.7 (Lane 2), THP-1 (Lane 3), K-562 (Lane 4), HeLa (Lane 5), HCT 116 (Lane 6), A549 (Lane 7), Mouse Spleen (Lane 8), Rat Spleen (Lane 9), Mouse Skeletal Muscle (Lane 10) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX), 12 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20,000) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).