LS-C776200

antibody from LSBio

Targeting: WIPI2 ATG18B, ATG21, CGI-50, DKFZP434J154, DKFZp686P02188, FLJ12979, FLJ14217, FLJ42984

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Enhanced validation)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C776200

Provider

LSBio

Product name

WIPI2 Antibody (RPE) LS-C776200

Antibody type

Polyclonal

Description

Peptide Affinity Purified

Reactivity

Human

Host

Rabbit

Storage

Store at -20°C.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of ~49.4 kDa WIPI2 protein using Rabbit Anti-WIPI2 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-WIPI2 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~49.4 kDa.

Immunocytochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WIPI2 Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WIPI2 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) WIPI2 Antibody (D) Composite.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WIPI2 Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WIPI2 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) WIPI2 Antibody (D) Composite.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry analysis using Rabbit Anti-WIPI2 Polyclonal Antibody. Tissue: Heart. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-WIPI2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.