ABIN1450025

antibody from antibodies-online

Targeting: WIPI2 ATG18B, ATG21, CGI-50, DKFZP434J154, DKFZp686P02188, FLJ12979, FLJ14217, FLJ42984

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

ABIN1450025

Provider

antibodies-online

Product name

anti-WD Repeat Domain, Phosphoinositide Interacting 2 (WIPI2) (N-Term) antibody

Antibody type

Polyclonal

Antigen

A 17 amino acid peptide near the amino terminus of human WIPI2.

Description

Affinity chromatography purified via peptide column

Reactivity

Human, Rat

Host

Rabbit

Epitope

N-Term

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/mL

Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.

Handling

Avoid repeated freezing and thawing.

References

Submitted references

Freeze-fracture replica immunolabelling reveals human WIPI-1 and WIPI-2 as membrane proteins of autophagosomes.

Proikas-Cezanne T, Robenek H
Journal of cellular and molecular medicine 2011 Sep;15(9):2007-10

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Mammalian Atg18 (WIPI2) localizes to omegasome-anchored phagophores and positively regulates LC3 lipidation.

Polson HE, de Lartigue J, Rigden DJ, Reedijk M, Urbé S, Clague MJ, Tooze SA
Autophagy 2010 May;6(4):506-22

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Diversity of WD-repeat proteins.

Smith TF
Sub-cellular biochemistry 2008;48:20-30

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Western blot analysis of WIPI2 in human testis tissue lysate with WIPI2 antibody Cat.-No AP55522PU-N at 1 ?g/ml.