GTX629971

antibody from GeneTex

Targeting: ROCK1 p160ROCK

Antibody data

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Validation data

ROCK1 protein structure - GTX629971 shown in red.

Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [GT261] (GTX629971) diluted at 1:2000.
KD Reagent Type: siRNA
Downregulation: >75%
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
KD Reagent Prod page: KD Reagent Prod page
Antibody Lot Number: 41484

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ROCK1 antibody [GT261] detects ROCK1 protein by Western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g HeLa whole cell lysate/extractC. 30 ?g HepG2 whole cell lysate/extract5 % SDS-PAGEROCK1 antibody [GT261] (GTX629971) dilution: 1:1000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ROCK1 antibody [GT261] detects ROCK1 protein by Western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extractB. 30 ?g GL261 whole cell lysate/extractC. 30 ?g NIH-3T3 whole cell lysate/extractD. 30 ?g BCL-1 whole cell lysate/extractE. 30 ?g Raw264.7 whole cell lysate/extractF. 30 ?g C2C12 whole cell lysate/extract5 % SDS-PAGEROCK1 antibody [GT261] (GTX629971) dilution: 1:1000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ROCK1 antibody [GT261] detects ROCK1 protein by Western blot analysis.A. 30 ?g PC-12 whole cell lysate/extractB. 30 ?g Rat-2 whole cell lysate/extract5 % SDS-PAGEROCK1 antibody [GT261] (GTX629971) dilution: 1:1000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Untreated (¡V) and treated (+) PC-12 whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [GT261] (GTX629971) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [GT261] (GTX629971) diluted at 1:2000.