Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-56596 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56596, RRID:AB_2182167
- Product name
- Mouse Monoclonal ROCK1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This mAb recognizes the cleaved C-terminus of ROCK-1. It does not recognize the full-length protein. Since ROCK-1 is cleaved by caspase-3 during apoptosis, this may be used for detection of apoptosis.
- Reactivity
- Human, Mouse, Rat, Canine, Rabbit, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Decorin modulates collagen I-stimulated, but not fibronectin-stimulated, migration of C2C12 myoblasts.
Rho kinase regulates phagocytosis, surface expression of GlcNAc, and Golgi fragmentation of apoptotic PC12 cells.
Rho kinase regulates fragmentation and phagocytosis of apoptotic cells.
Anoxia and reoxygenation of human endothelial cells decrease ceramide glucosyltransferase expression and activates caspases.
Goetsch KP, Kallmeyer K, Niesler CU
Matrix biology : journal of the International Society for Matrix Biology 2011 Mar;30(2):109-17
Matrix biology : journal of the International Society for Matrix Biology 2011 Mar;30(2):109-17
Rho kinase regulates phagocytosis, surface expression of GlcNAc, and Golgi fragmentation of apoptotic PC12 cells.
Orlando KA, Pittman RN
Experimental cell research 2006 Oct 15;312(17):3298-311
Experimental cell research 2006 Oct 15;312(17):3298-311
Rho kinase regulates fragmentation and phagocytosis of apoptotic cells.
Orlando KA, Stone NL, Pittman RN
Experimental cell research 2006 Jan 1;312(1):5-15
Experimental cell research 2006 Jan 1;312(1):5-15
Anoxia and reoxygenation of human endothelial cells decrease ceramide glucosyltransferase expression and activates caspases.
Zhao H, Miller M, Pfeiffer K, Buras JA, Stahl GL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2003 Apr;17(6):723-4
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2003 Apr;17(6):723-4
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: cleaved ROCK1 Antibody (154C1465) [NB100-56596] - Analysis using Azide Free version of NB100-56596. Cleaved ROCK-1 in A) untreated, B) 1 hour and C) 4 hour staurosporin-treated (2 uM) Jurkat cells using this antibody. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used
