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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Western blot [2]
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- Product number
- NB100-624 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-624, RRID:AB_10001772
- Product name
- Rabbit Polyclonal ROCK1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. NB100-624 is specific for human Rho Kinase Beta protein.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Comprehensive proteome analysis of human skeletal muscle in cachexia and sarcopenia: a pilot study.
Hypoxia-inducible factors mediate coordinated RhoA-ROCK1 expression and signaling in breast cancer cells.
Activation of Rho-associated coiled-coil protein kinase 1 (ROCK-1) by caspase-3 cleavage plays an essential role in cardiac myocyte apoptosis.
Ebhardt HA, Degen S, Tadini V, Schilb A, Johns N, Greig CA, Fearon KCH, Aebersold R, Jacobi C
Journal of cachexia, sarcopenia and muscle 2017 Aug;8(4):567-582
Journal of cachexia, sarcopenia and muscle 2017 Aug;8(4):567-582
Hypoxia-inducible factors mediate coordinated RhoA-ROCK1 expression and signaling in breast cancer cells.
Gilkes DM, Xiang L, Lee SJ, Chaturvedi P, Hubbi ME, Wirtz D, Semenza GL
Proceedings of the National Academy of Sciences of the United States of America 2014 Jan 21;111(3):E384-93
Proceedings of the National Academy of Sciences of the United States of America 2014 Jan 21;111(3):E384-93
Activation of Rho-associated coiled-coil protein kinase 1 (ROCK-1) by caspase-3 cleavage plays an essential role in cardiac myocyte apoptosis.
Chang J, Xie M, Shah VR, Schneider MD, Entman ML, Wei L, Schwartz RJ
Proceedings of the National Academy of Sciences of the United States of America 2006 Sep 26;103(39):14495-500
Proceedings of the National Academy of Sciences of the United States of America 2006 Sep 26;103(39):14495-500
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ROCK1 Antibody [NB100-624] - Detection of human and mouse Rho Kinase Beta (ROCK1/p160ROCK) on HeLa whole cell lysate using NB100-624. Rho Kinase Beta was also IPed using NB100-622 and NB100-623 at 3 ug/mg lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ROCK1 Antibody [NB100-624] - Detection of human and mouse ROCK1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ROCK1 antibody NB100-624 (lot NB100-624-2) used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. ROCK1 was also immunoprecipitated by a previous lot (lot NB100-624-1) of this antibody. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).
