MAB10497

antibody from Abnova Corporation

Targeting: AURKA AIK, ARK1, AurA, BTAK, PPP1R47, STK15, STK6, STK7

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB10497

Provider

Abnova Corporation

Proper citation

Product name

AURKA monoclonal antibody, clone 1F8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against partial recombinant AURKA.

Isotype

IgG

Antibody clone number

1F8

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis using AURKA monoclonal antibody, clone 1F8 (Cat # MAB10497) against HEK293 (1), SW-620 (2), MCF-7 (3), Jurkat (4), HeLa (5), HepG2 (6), COS-7 (7) and PC-12 (8) cell lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

ELISA detection with AURKA monoclonal antibody, clone 1F8 (Cat # MAB10497).

Validation comment

ELISA

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using AURKA monoclonal antibody, clone 1F8 (Cat # MAB10497) (green). Blue: DRAQ5 fluorescent DNA dye.

Validation comment

Immunofluorescence

Flow cytometry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Flow cytometric analysis of K-562 cells using AURKA monoclonal antibody, clone 1F8 (Cat # MAB10497) (green) and negative control (purple).

Validation comment

Flow Cytometry