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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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Validation data
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- Product number
- LS-C204509 - Provider product page
- Provider
- LSBio
- Product name
- AURKA / Aurora-A Antibody (clone 3H1) LS-C204509
- Antibody type
- Monoclonal
- Description
- Protein G purified
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3H1
- Storage
- Store at 4°C or -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western analysis of AURKA / Aurora-A antibody. Blot of HeLa cells treated with 100 ng/mL of nocodazole for 18 hours was probed with AURKA / Aurora-A antibody. Nocodazole is a microtubule inhibitor which induces cells G2/M phase and induces Aurora A expression. The AURKA / Aurora-A antibody monoclonal binds strongly and cleanly to a band at about 46 kDa.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Shows HeLa cell cultures stained with AURKA / Aurora-A antibody antibody (green). Aurora A localizes in spindle poles and mitotic spindles at anaphase and concentrates on the midbody between the two daughter cells during telophase. Counterstained is a chicken polyclonal antibody against Vimentin (red). Blue is a DNA stain.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Shows HeLa cell cultures stained with AURKA / Aurora-A antibody antibody (green). Aurora A localizes in spindle poles and mitotic spindles at anaphase and concentrates on the midbody between the two daughter cells during telophase. Counterstained is a chicken polyclonal antibody against Vimentin (red). Blue is a DNA stain.
