ABIN1842248
antibody from antibodies-online
Targeting: AURKA
AIK, ARK1, AurA, BTAK, PPP1R47, STK15, STK6, STK7
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- ABIN1842248 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Aurora Kinase A (AURKA) antibody
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Rat, Bovine, Porcine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5A12
- Vial size
- 100 μL
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western analysis of MCA-3H1 in HeLa cells. Blot of HeLa cells treated with 100 ng/mL nocodazole for 18 hours was probed with MCA-3H1. Nocodazole is microtubule inhibitor which induces cells G2/M phase and induces aurora A expression. The MCA-3H1 monoclonal binds strongly to a band at about 46 kDa: aurora A and weakly to a band at 38 kDa: aurora B. Middle: Blot of expressed human aurora A, B and C was probed with MCA-3H1. This antibody reacted with aurora A at 46kDa and aurora B at 38 kDa.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- shows HeLa cell cultures stained with MCA-3H1 antibody (green). Aurora A localizes in spindle poles and mitotic spindles at anaphase, while aurora B localizes in midzone at metaphase and concentrates on the midbody between the two daughter cells during telephase. Counterstained is our chicken polyclonal antibody against Vimentin: CPCA-Vim (red). Blue is a DNA stain.