ABIN1842248

antibody from antibodies-online

Targeting: AURKA AIK, ARK1, AurA, BTAK, PPP1R47, STK15, STK6, STK7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ABIN1842248

Provider

antibodies-online

Product name

anti-Aurora Kinase A (AURKA) antibody

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Rat, Bovine, Porcine

Host

Mouse

Isotype

IgG

Antibody clone number

5A12

Vial size

100 μL

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Western analysis of MCA-3H1 in HeLa cells. Blot of HeLa cells treated with 100 ng/mL nocodazole for 18 hours was probed with MCA-3H1. Nocodazole is microtubule inhibitor which induces cells G2/M phase and induces aurora A expression. The MCA-3H1 monoclonal binds strongly to a band at about 46 kDa: aurora A and weakly to a band at 38 kDa: aurora B. Middle: Blot of expressed human aurora A, B and C was probed with MCA-3H1. This antibody reacted with aurora A at 46kDa and aurora B at 38 kDa.

Immunocytochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

shows HeLa cell cultures stained with MCA-3H1 antibody (green). Aurora A localizes in spindle poles and mitotic spindles at anaphase, while aurora B localizes in midzone at metaphase and concentrates on the midbody between the two daughter cells during telephase. Counterstained is our chicken polyclonal antibody against Vimentin: CPCA-Vim (red). Blue is a DNA stain.