LS-C286340

antibody from LSBio

Targeting: AURKA AIK, ARK1, AurA, BTAK, PPP1R47, STK15, STK6, STK7

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C286340

Provider

LSBio

Product name

AURKA / Aurora-A Antibody (phospho-Thr288) LS-C286340

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at 2-8°C for up to one year.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Human Phospho-Aurora A (T288) by Immunocytochemistry. Samples: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Phospho-Aurora A (T288) used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Human Phospho-Aurora A (T288) by Immunocytochemistry. Samples: NBF-fixed asynchronous HeLa cells. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-Aurora A. Antibody: Affinity purified rabbit anti-Phospho-Aurora A (T288) used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human Phospho-Aurora A (T288) by Immunocytochemistry. Samples: NBF-fixed asynchronous HeLa cells. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-Aurora A. Antibody: Affinity purified rabbit anti-Phospho-Aurora A (T288) used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human Phospho-Aurora A (T288) by Immunocytochemistry. Samples: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Phospho-Aurora A (T288) used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human Phospho-Aurora A (T288) by Immunocytochemistry. Samples: NBF-fixed asynchronous HeLa cells. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-Aurora A. Antibody: Affinity purified rabbit anti-Phospho-Aurora A (T288) used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.