AP17096PU-N
antibody from Acris Antibodies GmbH
Targeting: AURKA
AIK, ARK1, AurA, BTAK, PPP1R47, STK15, STK6, STK7
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP17096PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP17096PU-N, RRID:AB_10685378
- Product name
- anti Aurora kinase A (N-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 110~139 amino acids from the N-terminal region of Human AIK
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- LEFT: Western blot analysis of AIK Antibody (N-term) Cat.-No AP17096PU-N in Hela cell line lysates (35ug/lane). AIK (arrow) was detected using the purified Pab. RIGHT: Western blot analysis of AIK (arrow) using rabbit polyclonal AIK Antibody (N-term) Cat.-No AP17096PU-N. 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the AIK gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human testis reacted with AIK Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of jurkat cells using AIK Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.