AM20208PU-N

antibody from Acris Antibodies GmbH

Targeting: AURKA AIK, ARK1, AurA, BTAK, PPP1R47, STK15, STK6, STK7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

AM20208PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM20208PU-N, RRID:AB_10758881

Product name

anti Aurora-A/B (C-term) (incl. pos. control)

Antibody type

Monoclonal

Antigen

Peptide conjugated to hemocyaninEpitope: C-terminus.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

5F11

Vial size

0.1 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Detection of endogenous Aurora-A/B: Whole cell extracts of vanadate treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The Immunoblot was probed with monoclonal Aurora-A/B antibody AM20208PU-N (5F11) (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).Lane 1: A431 Lane 2: SW480 Lane 3: SW620 Lane 4: HT29Lane 5: MCF-7 Lane 6: MDA-MB231 Lane 7: T47D