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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX85609 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX85609, RRID:AB_10723630
- Product name
- Aurora A (phospho Thr288) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Storage
- +4¢XC or -20¢XC, Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Panel A: Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) were immunoprecipitated with a pan-Aurora A monoclonal antibody (clone 35C1), resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal antibody against Thr288 phosphorylated Aurora A (Poly6187). Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.?
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Panel B: The blot shown in Panel A was stripped and re-probed with pan-Aurora specific polyclonal antibody (Poly6033) to verify equal loading of Aurora A in the extracts. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Overnight nocodazole treated Hela cells stained with purified rabbit polyclonal antibody against Thr288 phosphorylated Aurora A, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG and DAPI.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Human Testis (formalin-fixed, paraffin-embedded) stained with AURKA antibody (GTX85609) at 1:50 followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.
