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LearnAM20207PU-N
antibody from OriGene
Targeting: AURKA
AIK, ARK1, AurA, BTAK, PPP1R47, STK15, STK6, STK7
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- AM20207PU-N - Provider product page
- Provider
- OriGene
- Product name
- Aurora A (AURKA) (N-term) (incl. pos. control) mouse monoclonal antibody, clone 7F12, Purified
- Antibody type
- Monoclonal
- Description
- Aurora A (AURKA) (N-term) (incl. pos. control) mouse monoclonal antibody, clone 7F12, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- AURKA
- Isotype
- IgG
- Antibody clone number
- 7F12
- Vial size
- 100 µg
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Detection of endogenous Aurora-A: Whole cell extracts of vanadate treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The Immunoblot was probed with monoclonal antibody AM20207PU-N (7F12) (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2: SW480 Lane 3: SW620 Lane 4: HT29 Lane 5: MCF-7 Lane 6: MDA-MB231 Lane 7: T47D
- Validation comment
- WB
