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LearnNBP2-50040
antibody from Novus Biologicals
Targeting: AURKA
AIK, ARK1, AurA, BTAK, PPP1R47, STK15, STK6, STK7
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- NBP2-50040 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Aurora A Antibody
- Antibody type
- Monoclonal
- Description
- Tissue culture supernatant. This antibody does not cross-react with Aurora B or Aurora C.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.5 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Aurora A Antibody (1A11) [NBP2-50040] - Left: Analysis of HeLa cells treated with 100ng/ml nocodazole for 18 h probed with NBP2-50040. Nocodazole is a microtubule depolymerizing agent which induces cells to halt at G2/M phase and also induces Aurora A expression. NBP2-50040 binds strongly and cleanly to a band at about 46 kDa. Right: Blot of recombinant full length human Aurora A, B and C proteins was probed with NBP2-50040.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Aurora A Antibody (1A11) [NBP2-50040] - Analysis of different cell lysates and recombinant protein solutions using mouse Aurora A mAb. Left: cells were treated with 100ng/mL of nocodazol (a microtubule depolymerizing agent which induces cells to halt at G2/M phase) for 6hrs: [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] Equine NBL6 cells, and [5] murine KR158 cells. The antibody apparently does not recognize canine or equine aurora A, but does bind human and murine. Right: equal amounts of purified human recombinant aurora A, B, C as indicated. The NBP2-50040 antibody reacts with the aurora A recombinant protein but not aurora B or C.
