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LearnMA5-15804
antibody from Invitrogen Antibodies
Targeting: AURKA
AIK, ARK1, AurA, BTAK, PPP1R47, STK15, STK6, STK7
Western blotAntibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- MA5-15804 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Aurora A Monoclonal Antibody (4G10)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15804 targets AURKA in IHC and WB applications and shows reactivity with Human, Non-human primate, and Rat samples.
- Antibody clone number
- 4G10
- Concentration
- Conc. Not Determined
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AURKA using AURKA monoclonal antibody (Product # MA5-15804) in HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), HeLa (5), HepG2 (6), COS-7 (7) and PC-12 (8) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on nuclear extracts (30 µg lysate) of HCT-116 (Lane 1), HCT-116 treated with Paclitaxel (100 nM, 18 hrs)(Lane 2), HT-29 (Lane 3), HT-29 treated with Paclitaxel (100 nM, 18 hrs) (Lane 4), SK-BR-3 (Lane 5), SK-BR-3 treated with Nocodazole (100 ng/mL, 16 hrs) (Lane 6). The blot was probed with Aurora A Monoclonal Antibody (4G10)(Product #MA51-5804, 1:2,000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4,000 dilution). A 48 kDa band corresponding to Aurora A was observed upon the indicated treatments in the cell lines. The bands marked with * at 40 kDa and 35k Da may represent Aurora B and C respectively, however, their identity could not be confirmed.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and lung cancer (right) using AURKA monoclonal antibody (Product # MA5-15804) followed with DAB staining.
