Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB503-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal CXCL16 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse CXCL16 in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, recombinant mouse CXCL1, 2, 6, 9, 10, 11, 12, 13, 14, 15, recombinant rat CXCL1, 2, 3, 5, or recombinant porcine CXCL8 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Chemotaxis Induced by CXCL16 and Neutralization by Mouse CXCL16 Antibody. Recombinant Mouse CXCL16 Chemokine Domain (Catalog # 503-CX) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CXCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL16 Chemokine Domain (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL16 Monoclonal Antibody (Catalog # MAB503). The ND50 is typically 0.15-0.6 µg/mL.