AF503

antibody from R&D Systems

Targeting: CXCL16 CXCLG16, SR-PSOX, SRPSOX

Western blot (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF503

Provider

R&D Systems

Product name

Mouse CXCL16 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse CXCL16 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CXCL16, rhFractalkine, recombinant mouse Fractalkine, and recombinant rat Fractalkine is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q8BSU2


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

IL-33 modulates inflammatory brain injury but exacerbates systemic immunosuppression following ischemic stroke.

Zhang SR, Piepke M, Chu HX, Broughton BR, Shim R, Wong CH, Lee S, Evans MA, Vinh A, Sakkal S, Arumugam TV, Magnus T, Huber S, Gelderblom M, Drummond GR, Sobey CG, Kim HA
JCI insight 2018 Sep 20;3(18)

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Comprehensive assessment of chemokine expression profiles by flow cytometry.

Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
The Journal of clinical investigation 2010 Mar;120(3):907-23

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Elevated urinary VCAM-1, P-selectin, soluble TNF receptor-1, and CXC chemokine ligand 16 in multiple murine lupus strains and human lupus nephritis.

Wu T, Xie C, Wang HW, Zhou XJ, Schwartz N, Calixto S, Mackay M, Aranow C, Putterman C, Mohan C
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 15;179(10):7166-75

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Elevated urinary VCAM-1, P-selectin, soluble TNF receptor-1, and CXC chemokine ligand 16 in multiple murine lupus strains and human lupus nephritis.

Wu T, Xie C, Wang HW, Zhou XJ, Schwartz N, Calixto S, Mackay M, Aranow C, Putterman C, Mohan C
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 15;179(10):7166-75

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Excreted urinary mediators in an animal model of experimental immune nephritis with potential pathogenic significance.

Wu T, Xie C, Bhaskarabhatla M, Yan M, Leone A, Chen SS, Zhou XJ, Putterman C, Mohan C
Arthritis and rheumatism 2007 Mar;56(3):949-59

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Intervention of MAdCAM-1 or fractalkine alleviates graft-versus-host reaction associated intestinal injury while preserving graft-versus-tumor effects.

Ueha S, Murai M, Yoneyama H, Kitabatake M, Imai T, Shimaoka T, Yonehara S, Ishikawa S, Matsushima K
Journal of leukocyte biology 2007 Jan;81(1):176-85

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Inhibition of angiogenesis by interleukin-4 gene therapy in rat adjuvant-induced arthritis.

Haas CS, Amin MA, Allen BB, Ruth JH, Haines GK 3rd, Woods JM, Koch AE
Arthritis and rheumatism 2006 Aug;54(8):2402-14

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Chemotaxis Induced by CXCL16 and Neutralization by Mouse CXCL16 Antibody. Recombinant Mouse CXCL16 Chemokine Domain (Catalog # 503-CX) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CXCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL16 Chemokine Domain (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF503). The ND₅₀ is typically 0.1-0.4 µg/mL.