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Western blot
Blocking/Neutralizing
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Other assay [2]
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- Product number
- MA5-23952 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CXCL16 Monoclonal Antibody (256213)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In Western blots, no cross-reactivity with recombinant human CXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12/SDF-1 alpha,12/SDF-1 beta, 13, recombinant mouse CXCL1, 2, 6, 9, 10, 12/SDF-1 alpha, 13, 16, recombinant rat CXCL1, 2, 3/CINC-2 alpha, 3/CINC-2 beta, recombinant canine CXCL8, or recombinant porcine CXCL8 is observed. Reconstitute at 0.5 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 256213
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of CXCL16 by Product # MA523952 showing the decrease in chemotaxis with increasing concentrations of anti CXCL16 monoclonal antibody (256213). Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of CXCL16 in BaF3 mouse pro-B cell line transfected with mouse CXCR6. Samples were incubated in CXCL16 monoclonal antibody (Product # MA5-23952). Recombinant Human CXCL16 Chemokine Domain chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CXCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CXCL16 Chemokine Domain (20 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCL16 Monoclonal Antibody. The ND50 is typically 2-8 µg/mL.
