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Western blot
Immunohistochemistry
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [2]
- Other assay [2]
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- Product number
- PA5-47977 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CXCL16 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In ELISAs, less than 0.05% cross-reactivity with recombinant mouse (rm) CXCL16, rm6Ckine, recombinant human (rh) BLC, rhCTACK, rhIL-8, rhPF4, and rhLymphotactin is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CXCL16 in immersion fixed paraffin-embedded sections of human lymph node. Samples were incubated in CXCL16 polyclonal antibody (Product # PA5-47977) using a dilution of 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CXCL16 in immersion fixed paraffin-embedded sections of human lymphoma. Samples were incubated in CXCL16 polyclonal antibody (Product # PA5-47977) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of CXCL16 in BaF3 mouse pro‚B cell line transfected with mouse CXCR6. Samples were incubated in CXCL16 polyclonal antibody (Product # PA5-47977). Recombinant Human CXCL16 Chemokine Domain induces chemotaxis in BaF3 mouse pro‚B cell line transfected with mouse CXCR6 in a dose-dependent manner (orange line). Chemotaxis elicited by Recombinant Human CXCL16 Chemokine Domain (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Primate CXCL16 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically0.05-0.25 μg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of CXCL16 in BaF3 mouse pro‚B cell line transfected with mouse CXCR6. Samples were incubated in CXCL16 polyclonal antibody (Product # PA5-47977). Recombinant Human CXCL16 Chemokine Domain induces chemotaxis in BaF3 mouse pro‚B cell line transfected with mouse CXCR6 in a dose-dependent manner (orange line). Chemotaxis elicited by Recombinant Human CXCL16 Chemokine Domain (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Primate CXCL16 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically0.05-0.25 μg/mL.
