MA5-27845

antibody from Invitrogen Antibodies

Targeting: CXCL16 CXCLG16, SR-PSOX, SRPSOX

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27845

Provider

Invitrogen Antibodies

Product name

CXCL16 Monoclonal Antibody (GT516)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: HeLa, HeLa membrane extract Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

GT516

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Dendritic cells maintain anti-tumor immunity by positioning CD8 skin-resident memory T cells.

Vella JL, Molodtsov A, Angeles CV, Branchini BR, Turk MJ, Huang YH
Life science alliance 2021 Oct;4(10)

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3. CD11c + cells express CXCL16 in melanoma-associated vitiligo (MAV)-affected skin. (A) Heat map showing gene expression of chemokines and receptors in CD11c + CD11b neg , CD11c + CD11b + , and CD8 + T cells sorted from skin of MAV-affected mice. Data presented as log2-normalized expression. (B) Surface expression of CXCL16 on CD11c + cells isolated from skin of naive (n = 3) or MAV-affected (n = 5) mice, skin-draining LNs, or spleen 7, 14, and 35 d after surgery. Representative of three independent experiments. (C) Surface expression of CXCL16 on CD45 + CD11c + , CD45 + CD11c neg , and CD45 neg cells isolated from skin of MAV-affected mice, 35 d after surgery. Representative of two independent experiments with skin collected between 35- and 60-d post-surgery. Dotted horizontal line indicates background CXCL16 expression in unstained cell sample. (D) Expression of CXCL16 and CD11c in unaffected and MAV-affected mice; CD11c (green), CXCL16 (red), and nuclei (blue). Scale bar, 50 mum. (E) CXCL16 expression in IF images from skin of unaffected and MAV-affected mice; n = 5-8 mice. (F) CXCL16 expression in hair follicles with or without CD11c + cell clusters in MAV-affected skin; n = 8 mice. (G) CD11c + and CXCL16 + cells in skin from MAV-affected patients. Stains identify CXCL16 (green) and CD11c (red); dark red identifies colocalization of CD11c and CXCL16. Scale bar, 25 mum. (H) Percent of CD11c + cells expressing CXCL16 in MAV-affected patient skin. (B, C, H) Symbols r