Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-30786 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CXCL16 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: CXCL16-transfected 293T, HeLa. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.24 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CXCL16 using 30 µg HeLa whole cell lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a CXCL16 polyclonal antibody (Product # PA5-30786) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CXCL16 using 30 µg of HepG2 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a CXCL16 polyclonal antibody (Product # PA5-30786) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CXCL16 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a CXCL16 Polyclonal Antibody (Product # PA5-30786) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CXCL16 Polyclonal Antibody (Product # PA5-30786). Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with CXCL16 Polyclonal Antibody (Product # PA5-30786) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CXCL16 in methanol-fixed A549 cells using a CXCL16 polyclonal antibody (Product # PA5-30786) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CXCL16 Polyclonal Antibody detects CXCL16 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse thymus gland. CXCL16 stained by CXCL16 Polyclonal Antibody (Product # PA5-30786) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CXCL16 Polyclonal Antibody detects CXCL16 protein at cytosol and membrane on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. CXCL16 Polyclonal Antibody (Product # PA5-30786) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.