PA5-30786

antibody from Invitrogen Antibodies

Targeting: CXCL16 CXCLG16, SR-PSOX, SRPSOX

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30786

Provider

Invitrogen Antibodies

Product name

CXCL16 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: CXCL16-transfected 293T, HeLa.

Concentration

1.24 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CXCL16 using 30 µg HeLa whole cell lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a CXCL16 polyclonal antibody (Product # PA5-30786) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CXCL16 using 30 µg of HepG2 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a CXCL16 polyclonal antibody (Product # PA5-30786) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CXCL16 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a CXCL16 Polyclonal Antibody (Product # PA5-30786) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CXCL16 Polyclonal Antibody (Product # PA5-30786). Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with CXCL16 Polyclonal Antibody (Product # PA5-30786) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of CXCL16 in methanol-fixed A549 cells using a CXCL16 polyclonal antibody (Product # PA5-30786) at a 1:500 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CXCL16 Polyclonal Antibody detects CXCL16 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse thymus gland. CXCL16 stained by CXCL16 Polyclonal Antibody (Product # PA5-30786) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CXCL16 Polyclonal Antibody detects CXCL16 protein at cytosol and membrane on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. CXCL16 Polyclonal Antibody (Product # PA5-30786) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.