Explore
Validate
Learn
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB976 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CXCL16 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CXCL16 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12/SDF-1 alpha ,12/SDF-1 beta , 13, recombinant mouse CXCL1, 2, 6, 9, 10, 12/SDF-1 alpha , 13, 16, recombinant rat CXCL1, 2, 3/CINC-2 alpha , 3/CINC-2 beta , recombinant canine CXCL8, or recombinant porcine CXCL8 is observed.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
NP_071342- Isotype
- IgG
- Antibody clone number
- 256213
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CXCL16 and Neutralization by Human CXCL16 Antibody. Recombinant Human CXCL16 Chemokine Domain (Catalog # 976-CX) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CXCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL16 Chemokine Domain (20 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCL16 Monoclonal Antibody (Catalog # MAB976). The ND50 is typically 2-8 µg/mL.
