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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Immunohistochemistry [1]
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- Product number
- PB9995 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Cdk6 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for CDK6 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. CDK6 information: Molecular Weight: 36938 MW; Subcellular Localization: Cytoplasm. Nucleus. Cell projection, ruffle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Localized to the ruffling edge of spreading fibroblasts. Kinase activity only in nucleus. Localized to the cytosol of neurons and showed prominent staining around either side of the nucleus (By similarity). Present in the cytosol and in the nucleus in interphase cells and at the centrosome during mitosis from prophase to telophase (PubMed:23918663); Tissue Specificity: Expressed ubiquitously. Accumulates in squamous cell carcinomas, proliferating hematopoietic progenitor cells, beta-cells of pancreatic islets of Langerhans, and neuroblastomas. Reduced levels in differentiating cells.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Role and mechanism of Circ-PDE7B in the formation of keloid.
Combined Anti-Cancer Effects of Platycodin D and Sorafenib on Androgen-Independent and PTEN-Deficient Prostate Cancer.
Triclosan down-regulates fatty acid synthase through microRNAs in HepG2 cells.
Effects of miR-1236-3p and miR-370-5p on activation of p21 in various tumors and its inhibition on the growth of lung cancer cells.
Tang Y, Li X
International wound journal 2023 Nov;20(9):3738-3749
International wound journal 2023 Nov;20(9):3738-3749
Combined Anti-Cancer Effects of Platycodin D and Sorafenib on Androgen-Independent and PTEN-Deficient Prostate Cancer.
Lu Z, Song W, Zhang Y, Wu C, Zhu M, Wang H, Li N, Zhou Y, Xu H
Frontiers in oncology 2021;11:648985
Frontiers in oncology 2021;11:648985
Triclosan down-regulates fatty acid synthase through microRNAs in HepG2 cells.
Sun D, Zhao T, Long K, Wu M, Zhang Z
European journal of pharmacology 2021 Sep 15;907:174261
European journal of pharmacology 2021 Sep 15;907:174261
Effects of miR-1236-3p and miR-370-5p on activation of p21 in various tumors and its inhibition on the growth of lung cancer cells.
Li C, Ge Q, Liu J, Zhang Q, Wang C, Cui K, Chen Z
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2017 Jun;39(6):1010428317710824
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2017 Jun;39(6):1010428317710824
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of Cdk6 using anti-Cdk6 antibody (PB9995). Cdk6 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cdk6 Antibody (PB9995) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image
