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Western blot
Proximity ligation assayAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00001021-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001021-M01, RRID:AB_537640
- Product name
- Mouse Monoclonal Cdk6 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CDK6 - cyclin-dependent kinase 6
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references MiR-26a and miR-138 block the G1/S transition by targeting the cell cycle regulating network in prostate cancer cells.
APRIL promotes cell-cycle progression in primary multiple myeloma cells: influence of D-type cyclin group and translocation status.
Erdmann K, Kaulke K, Rieger C, Salomo K, Wirth MP, Fuessel S
Journal of cancer research and clinical oncology 2016 Nov;142(11):2249-61
Journal of cancer research and clinical oncology 2016 Nov;142(11):2249-61
APRIL promotes cell-cycle progression in primary multiple myeloma cells: influence of D-type cyclin group and translocation status.
Quinn J, Glassford J, Percy L, Munson P, Marafioti T, Rodriguez-Justo M, Yong K
Blood 2011 Jan 20;117(3):890-901
Blood 2011 Jan 20;117(3):890-901
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cdk6 Antibody (8H4) [H00001021-M01] - Analysis of CDK6 expression in transfected 293T cell line by CDK6 monoclonal antibody (M01), clone 8H4.Lane 1: CDK6 transfected lysate(36.9 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cdk6 Antibody (8H4) [H00001021-M01] - CDK6 monoclonal antibody (M01), clone 8H4. Analysis of CDK6 expression in PC-12.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Cdk6 Antibody (8H4) [H00001021-M01] - Detection limit for recombinant GST tagged CDK6 is 0.3 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Cdk6 Antibody (8H4) [H00001021-M01] - Analysis of monoclonal antibody to CDK6 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cdk6 Antibody (8H4) [H00001021-M01] - Analysis of monoclonal antibody to CDK6 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
