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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- GTX48870 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX48870, RRID:AB_11173214
- Product name
- Raf1 (phospho Ser259) antibody
- Antibody type
- Polyclonal
- Host
- Rabbit
- Vial size
- 100 µl
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- GeneTex Goat anti antibody (GTX48870) was used to detect purified Beta Phospho Glucomutase under reducing (R) and non-reducing (NR) conditions. Reduced samples of protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in blocking buffer). Detection shown was using Dylight 488 conjugated Donkey anti goat (1:10K in TBS/blocking buffer 1 hr RT).
- Validation comment
- WB
